e22c-gal4 – Utmkb

FlyBase: a database for drosophila genetics and molecular biology Drives expression in multiple stages/tissues, including in the embryonic ectoderm and epidermis, in the larval brain, ubiquitously in imaginal discs, and in follicle cells during oogenesis.

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A. E. Anderson & M. J. Galko Epigenetic Reporters in Fly Wound Healing Figure 1. Reporters for seven proteins are downregulated in the vicinity of the wound. (A)−(G ) Dissected epidermal whole mounts of larvae heterozygous for e22c-Gal4, UAS-DsRed2-Nuc and the

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Additional file 3: Figure S3. crc regulates genes involved in translation including 4E-BP. (A, B) KEGG pathway analysis performed on microarray data HA-crcA.pMT-Puro S2 stable cells relative to HA.pMT-Puro S2 stable cells, each treated with 0.7 mM CuSO 4 for 3 h or 6 h to identify pathways significantly enriched within the list of differentially expressed up- or down-regulated genes with fold

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News Oct 2019: Imported about 5000 data points from Publication “An RNAi Screen for Genes Required for Growth of Drosophila Wing Tissue” Sep 2019: Updated FlyBase

31/8/2015 · that e22c-Gal4 and pannier-Gal4, but not A58-Gal4, exhibit early tendon cell expression that is later lost (Figures S2A-S2F). To test if early expression of PINCH RNAi in tendon cells contributed to epidermal syncytium formation we performed).

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中国细胞生物学学报 698 · 研究简报· 原区。这类细胞来源于滤泡干细胞(follicle stem cell, FSC), 随着包裹的完成分化为极细胞前体(prepolar cell)和滤泡上皮细胞(epithelial follicle cell)。前者立即退出有丝分裂, 于第1期卵室时受生殖细胞诱导,

1/4/2005 · (B) Driving the UAS-nkd transgene ubiquitously with E22C-Gal4 significantly rescues the cuticle pattern defect, to a pattern much more closely resembling that of wild type (C). The UAS-nkd and the E22C-Gal4 transgenes were independently recombined onto the

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4/3/2013 · Calcium Flashes Orchestrate the Wound Inflammatory Response through DUOX Activation and Hydrogen Peroxide Release Movie showing resolution of the calcium flash in an w;e22c-Gal4,UAS-GCaMP3,UAS-mCherry-moesin embryo after wounding (red is

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When we expressed wild-type Abl ubiquitously, using either e22c-GAL4 or arm-GAL4:VP16, it led to embryonic lethality, although the penetrance of this lethality was lower with e22c-GAL4 (Table 1), suggesting that embryos can tolerate reasonably high levels of).

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15/10/2003 · H5F7 staining of embryos expressing UAS-nrvX transgenes revealed that for the btl-Gal4 and e22c-Gal4 drivers, nrv2.1, nrv2.2 and nrv3 had equivalent staining levels and subcellular localizations, but that although detectable, nrv1 staining was significantly weaker.

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To visualize live actin dynamics during wound closure, we used the e22c-GAL4 driver to express a GFP–actin fusion protein in all epithelial cells. Time-lapse confocal movies demonstrated that

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As expected, the combination of the e22c-GAL4 with UAS-trpml did not improve adult viability (Figure 6A). To our surprise, the hemocyte/fat body and glial drivers restored normal survival, climbing activity, and synaptic transmission (Figures 6A–6D).

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7/5/2018 · (B) Confocal microscopy images of z projections of fixed lateral stage 12 embryos from control (con) embryos and those in which RNAi against veli is driven by the e22c-GAL4 ectodermal driver. Macrophages are labeled in red by the expression of . The white

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Compared to the A58-GAL4-reporter, a higher percentage of open wounds was obtained for 16 RNAi lines with the e22c-GAL4-reporter and for 13 lines with the UAS-Dcr-2; A58-GAL4-reporter. However, this latter driver also showed a pronounced increase in

15/10/2019 · All crosses were raised at 25 C unless indicated. The GAL4/UAS system was used to drive tissue-specific gene expression of transgenes under UAS control (Brand and Perrimon, 1993). For the embryonic and larval epidermis, e22c-Gal4 was used (Lawrence et

Author: Chang Ru Tsai, Michael J. Galko

Migrating cells penetrate tissue barriers during development, inflammatory responses, and tumor metastasis. We study if migration in vivo in such three-dimensionally confined environments requires changes in the mechanical properties of the surrounding cells using embryonic Drosophila melanogaster hemocytes, also called macrophages, as a model.

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lethal, with Scer\GAL4 e22c, Scer\GAL4 sqh.PW, nAChRα5 KK109791 ( Tsubouchi et al., 2012 ) lethal , with Scer\GAL4 e22c , Scer\GAL4 sqh.PW , nAChRα6 KK103877

12/12/2004 · GAL4 drivers MS1096, 71B, prd–GAL4, ptc–GAL4 and E22C–GAL4 were generally used at 29 C. Clones of cells expressing GAL4 from an actin promoter were

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da-Gal4 is expressed ubiquitously 60, e22c-Gal4 in the epidermis from embryonic stage 10–11 onward 59, and A58-Gal4 in the epidermis from the first larval instar onward 12.

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Negative regulation of Armadillo, a Wingless effector in Drosophila Li-Mei Pai1, Sandra Orsulic1,*, Amy Bejsovec2 and Mark Peifer1, e22c-GAL4/+females to armS10homozygous males. Second and

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pronounced with the e22c-Gal4 and Dcr-2;A58-Gal4 reporters, with weaker knockdown observed with the A58-Gal4 reporter. Scale bar 100 μm. C. Lesch et al. 3 SI FIGURE S2. —Quantification of wound closure with alternative RNAi lines, dominant-negative

E22C–Gal4 and 69B–Gal4 driver lines were used for homogeneous expression throughout the epidermis, en–Gal4 and ptc–Gal4 were used for expression in the corresponding rows of epidermal cells.

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We used the GAL4/UAS system (B rand and P errimon 1993) for tissue-specific expression of transgenes: A58-Gal4 (G alko and K rasnow 2004) and UAS-Dcr-2;A58-Gal4 (see below) express Gal4 predominantly in the larval epidermis, while e22c-Gal4 (L et al.

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15/7/2006 · e22c-Gal4 (D-F) and LE-Gal4 (G-I) were used to express DN-msn in the whole epidermis or in marginal cells of the epidermis, respectively. (E,F) and (H,I) show myosin 2/Armadillo co-staining. Arrowheads point at areas of disrupted myosin 2 staining correlating

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1/10/2003 · UAS transgene-bearing females were crossed with E22C-Gal4 males to drive high ubiquitous transgene expression in the resulting embryos. Flies were reared on standard cornmeal-agar-molasses medium and eggs were collected on apple juice-agar plates.

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The calcium sensor, GCamP3 (green), is ubiquitously expressed in the epidermis (e22c-Gal4, UAS-GCaMP3). In the larval epidermis soon after wounding, a calcium wave spreads from the wounded area.

職稱: 37.27

The e22c Gal4 driver (Duffy et al., 1998) was obtained from the Bloomington Drosophila Stock Center. For clonal analysis, cher EP(3)3175, cher Δ5 and cher Q1042X mutations were recombined onto a FRT 82B-containing chromosome.

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1/11/2010 · We used the GAL4/UAS system (B rand and P errimon 1993) for tissue-specific expression of transgenes: A58-Gal4 (G alko and K rasnow 2004) and UAS-Dcr-2;A58-Gal4 (see below) express Gal4 predominantly in the larval epidermis, while e22c-Gal4 (L et al.

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When crossed to the e22c-Gal4 or 69B-Gal4 drivers, only UAS-bsk DN and UAS-puc gave a strong dorsal closure defect like JNK pathway mutants. To express Basket DN in larval epidermis, female w 1118, UAS-bsk DN, UAS-bsk DN /w 1118; msn-lacZ, A58 w

The GAL4/UAS system was used to drive tissue-specific gene expression of transgenes under UAS control (Brand and Perrimon, 1993). For the embryonic and larval epidermis, e22c-Gal4 was used (Lawrence et al., 1995); for the larval epidermis, A58-Gal4 ).

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e22c-GAL4/+females to armS10homozygous males. Second and third, we tested armS10 in embryos with a maternal and zygotic con-tribution composed entirely of XP33or armarmH8.6mutant protein. Germline clones of armXP33or armH8.6 were generated as

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15/1/2008 · In Br overexpression experiments, the UAS-Br-Z1 construct (a gift from L. Riddiford) was driven by the Gal80-Gal4, CY2-Gal4 expression system (Suster et al., 2004). Mosaic analysis of the effects of br-cells were done with the br CJ89 FRT 19A /FRT 19A; e22c).

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8/10/1998 · UAS-dTcf-ΔN expressed ubiquitously with E22C-GAL4 produces b, moderate to c, severe segment polarity phenotypes. d, Normal En antibody staining in

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Lineage tracing using a Flp-out Gal4 cassette (see Experimental Procedures) determined that e22c-Gal4 and pannier-Gal4, but not A58-Gal4, exhibit early tendon cell expression that is

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Nonsense-mediated mRNA decay (NMD) is a cellular surveillance mechanism that degrades transcripts containing premature translation termination codons, and it also influences expression of certain wild-type transcripts. Although the biochemical mechanisms of

1/3/2012 · Drosophila stocks Follicle cell clones were generated as previously described (Duffy et al. 1998) using the e22c-Gal4 driver and enhanced GFP under the control of the ubiquitin promoter (ubi-eGFP) to mark wild-type cells.Mutant alleles used for complementation and

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24/10/2008 · To study the effect of dSTIM RNAi during embryogenesis we used the da.Gal4 and e22c.Gal4 drivers to induce ubiquitous expression of RNAi in the embryo. This resulted in late 2 nd to early 3 rd instar larval lethality, with considerable variability between the

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Wound healing recapitulates morphogenesis in Drosophila embryos Article (PDF Available) in Nature Cell Biology 4(11):907-12 · December 2002 with 164 Reads How we measure ‘reads

5/12/2012 · Expression pattern of different GAL4 driver lines. The GAL4 driver lines were tested for the expression of UAS-GFP. A) The e22c-GAL4 line drives GFP expression in the follicular stem cells and thus in all FCs, albeit patchy.B) Slbo-GAL4 expresses strongly in border cells, in stretched FCs and in columnar FCs at the dorsal anterior side (centripetal FCs) and the posterior end and also in

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28/11/2008 · As expected, the combination of the e22c-GAL4 with UAS-trpml did not improve adult viability (Figure 6A). To our surprise, the hemocyte/fat body and glial drivers restored normal survival, climbing activity, and synaptic transmission (Figures 6A–6D).

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The calcium sensor, GCamP3 (green), is ubiquitously expressed in the epidermis (e22c-Gal4, UAS-GCaMP3). In the | Find, read and cite all the research you need on ResearchGate We use cookies to

Here, the FLP gene, which is under the control of upstream activator sequences (UAS>FLP), is driven by the Gal4 line e22c-Gal4 that is expressed in follicle stem cells. Late clones were induced by driving FLP under the control of a heat shock promoter (hsFLP;

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Homozygous UAS-DN-msn ﬂies were crossed with e22c-Gal4, LE-Gal4 or AS-Gal4 ﬂies to express DN-msn in the whole epidermis, marginal cells of the epidermis, or the amnioserosa, respectively. Identiﬁcation of msn genes and phylogenetic analysis

2/3/2006 · Conversely, serotonin and histamine, but not EGF, stimulate AC activity in flies expressing a GRD deletion (ΔGRD2) or a C-terminal fragment (Cterm) alone. (D) Pupal length is increased in flies expressing normal hNF1 using elav-Gal4 or e22c-Gal4 driversNf1

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These effects were not an indiscriminate consequence of raising the activity level of Wg. Driving ubiquitous Dfz2 overexpression with E22C–Gal4 or arm–VP16–Gal4 (data not shown) had no effect on epidermal patterning in wg null mutant embryos (Fig. 1a) nor in

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Use of FLP/FRT system to study Drosophila development Article · Literature Review (PDF Available) 1998) utilizing: FRT 19A BRK M68 / FRT 19A ubiGFP ; e22c-GAL4 UAS-flp. A UAS-BRK (a gift from

1/8/2018 · The nonsense-mediated messenger RNA (mRNA) decay (NMD) pathway is a cellular quality control and post-transcriptional gene regulatory mechanism and is essential for viability in most multicellular organisms . A complex of proteins has been identified to be

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